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Protocol A: Annexin V staining. Materials. • 12 x 75 mm round-bottom tubes. • 1X PBS. • Annexin V Apoptosis Detection kit (cat. nos. 1. Pellet cells, including any cells in supernatants from adherent cell cultures. · 2. Resuspended in ml of Becton Dickinson (BD) Annexin V Binding Buffer . Annexin V luciferase fusion proteins supplied in the assay reagent bind to PS during early apoptosis and are detected with a simple luminescence signal. The.

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In my experience, it is possible to fix annexin V stained cells and see great results on the flow. Stain cells with annexin V (in calcium-rich binding. As a new method for detecting apoptosis, the Annexin V binding assay is based on the measurement of the loss of plasma membrane asymmetry. 3. Observe cell staining with fluorescence microscopy · Cells that have bound annexin V-FITC will show green staining in the plasma membrane. · Cells that have.

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1. Wash cells twice with cold BioLegend cell staining buffer (Cat. No. · 2. Transfer µL of cell suspension in 5 ml test tube. · 3. Add 5 µL of FITC Annexin V. ASSAY PROTOCOL. NOTES. • Annexin V FITC and Propidium Iodide are light sensitive. All staining procedures must be performed without direct exposure to. As a new method for detecting apoptosis, the Annexin V binding assay is based on the measurement of the loss of plasma membrane asymmetry.